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Home > Product > ELISA Kit > Human 40S Ribosomal Protein S23, RPS23 ELISA Kit

Human 40S Ribosomal Protein S23, RPS23 ELISA Kit

Human 40S Ribosomal Protein S23, RPS23 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.55ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 3.55ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

40S Ribosomal Protein S23

Code

E7278Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.55ng/L

Detection range

7-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed: 28257692, PubMed: 23636399, PubMed: 25957688, PubMed: 25901680). The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of Peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel (PubMed: 23636399, PubMed: 25957688, PubMed: 25901680). Plays an important role in translational accuracy (PubMed: 28257692).

UniProt accession

MASS(Da)

15,808

GeneID

6228

Synonyms

40S ribosomal protein S23;RPS 23;RPS23;Small ribosomal subunit protein uS12

Gene names

RPS23

Research area

Epigenetics and Nuclear Signaling

Target protein

RPS23

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E7278Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 2.164 2.208 2.148
2.251
400ng/L 1.432 1.383 1.323
1.333
200ng/L 0.773 0.79 0.73
0.807
100ng/L 0.46 0.43 0.37
0.399
50ng/L 0.277 0.294 0.235
0.311
0ng/L 0.073 0.059 0
0.046

Precision

We measured random samples of E7278Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 500 16.57 3.3
Intra-Assay 2 18 174.2 11.44 6.6
Intra-Assay 3 18 364.2 15.79 4.3

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E7278Hu

    $320.00

  • 96T

    E7278Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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