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Home > Product > ELISA Kit > Human Sh3Kbp1 Binding Protein 1, SHKBP1 ELISA Kit

Human Sh3Kbp1 Binding Protein 1, SHKBP1 ELISA Kit

Human Sh3Kbp1 Binding Protein 1, SHKBP1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 12.14ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 12.14ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Sh3Kbp1 Binding Protein 1

Code

E7316Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

12.14ng/L

Detection range

20-3800ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Inhibits CBL-SH3KBP1 complex mediated down-regulation of EGFR signaling by sequestration of SH3KBP1. Binds to SH3KBP1 and prevents its interaction with CBL and inhibits translocation of SH3KBP1 to EGFR containing vesicles upon EGF stimulation.

UniProt accession

MASS(Da)

76,344

GeneID

92799

Synonyms

SETA-binding protein 1;SH3KBP1-binding protein 1;SHKBP 1;SHKBP1

Gene names

SHKBP1

Research area

Neuroscience

Target protein

SHKBP1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E7316Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 2.243 2.19 2.145
2.137
1000ng/L 1.385 1.313 1.268
1.242
500ng/L 0.766 0.81 0.765
0.854
250ng/L 0.482 0.506 0.461
0.529
125ng/L 0.191 0.187 0.142
0.184
0ng/L 0.056 0.045 0
0.035

Precision

We measured random samples of E7316Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1423.1 55.03 3.9
Intra-Assay 2 18 799.6 46.69 5.8
Intra-Assay 3 18 244.2 16.92 6.9

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E7316Hu

    $320.00

  • 96T

    E7316Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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