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Home > Product > ELISA Kit > Mouse Interleukin 1Α, IL-1A ELISA Kit

Mouse Interleukin 1Α, IL-1A ELISA Kit

Mouse Interleukin 1Α, IL-1A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.53ng/L

Detection range: 1-300ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.53ng/L

Detection range: 1-300ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Interleukin 1alpha

Code

E0045Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.53ng/L

Detection range

1-300ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

UniProt accession

MASS(Da)

31,023

GeneID

16175

Synonyms

IL 1 alpha;IL 1A;IL-1 alpha;IL1A;Interleukin-1 alpha

Gene names

IL1A

Research area

Immunology; Cancer

Target protein

IL1A

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0045Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
160ng/L 1.864 1.876 1.83
1.887
80ng/L 0.908 0.9 0.854
0.892
40ng/L 0.522 0.472 0.426
0.421
20ng/L 0.145 0.181 0.135
0.218
10ng/L 0.126 0.108 0.062
0.09
0ng/L 0.036 0.046 0
0.056

Precision

We measured random samples of E0045Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 116.9 3.62 3.1
Intra-Assay 2 18 49.9 2.31 4.6
Intra-Assay 3 18 70.5 2.94 4.2

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0045Mo

    $320.00

  • 96T

    E0045Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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