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Home > Product > ELISA Kit > Mouse Osteoprotegerin, OPG ELISA Kit

Mouse Osteoprotegerin, OPG ELISA Kit

Mouse Osteoprotegerin, OPG ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.45ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 5.45ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Osteoprotegerin

Code

E0110Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.45ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.

UniProt accession

MASS(Da)

45,923

GeneID

18383

Synonyms

Osteoclastogenesis inhibitory factor;Osteoprotegerin;TNFRSF 11B;TNFRSF11B;Tumor necrosis factor receptor superfamily member 11B

Gene names

TNFRSF11B

Research area

Cell Biology

Target protein

TNFRSF11B

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0110Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 1.832 1.882 1.845
1.933
600ng/L 1.053 1 0.963
0.948
300ng/L 0.558 0.529 0.491
0.499
150ng/L 0.224 0.234 0.197
0.244
75ng/L 0.15 0.151 0.114
0.152
0ng/L 0.035 0.037 0
0.039

Precision

We measured random samples of E0110Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 936.2 28.57 3.1
Intra-Assay 2 18 679.6 29.84 4.4
Intra-Assay 3 18 348.1 14.18 4.1

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E0110Mo

    $320.00

  • 96T

    E0110Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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