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Home > Product > ELISA Kit > Mouse Angiopoietin 1, ANG-1 ELISA Kit

Mouse Angiopoietin 1, ANG-1 ELISA Kit

Mouse Angiopoietin 1, ANG-1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.27ng/mL

Detection range: 0.5-150ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.27ng/mL

Detection range: 0.5-150ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Angiopoietin 1

Code

E0335Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.27ng/mL

Detection range

0.5-150ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme (By similarity).

UniProt accession

MASS(Da)

57,519

GeneID

11600

Synonyms

ANG 1;ANG-1;Angiopoietin-1

Gene names

ANG-1

Research area

Cardiovascular; Cancer

Target protein

ANG-1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0335Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
80ng/mL 2.231 2.272 2.219
2.313
40ng/mL 1.296 1.271 1.218
1.245
20ng/mL 0.82 0.758 0.705
0.696
10ng/mL 0.462 0.462 0.409
0.462
5ng/mL 0.181 0.222 0.169
0.262
0ng/mL 0.059 0.053 0
0.046

Precision

We measured random samples of E0335Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 52 1.55 3
Intra-Assay 2 18 17.5 0.9 5.2
Intra-Assay 3 18 23.5 1.26 5.4

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0335Mo

    $320.00

  • 96T

    E0335Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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