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Home > Product > ELISA Kit > Mouse Granzymes B, GZMS-B ELISA Kit

Mouse Granzymes B, GZMS-B ELISA Kit

Mouse Granzymes B, GZMS-B ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.26ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 3.26ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Granzymes B

Code

E0437Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.26ng/L

Detection range

7-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

UniProt accession

MASS(Da)

27,470

GeneID

14939

Synonyms

CCP1;CTLA-1;Cytotoxic cell protease 1;Fragmentin-2;Granzyme B(G,H;GZMB

Gene names

GZMB

Research area

Cell Biology

Target protein

GZMB

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0437Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 2.024 2.033 2.009
2.042
400ng/L 1.442 1.417 1.393
1.392
200ng/L 0.729 0.729 0.704
0.728
100ng/L 0.365 0.397 0.372
0.428
50ng/L 0.158 0.172 0.147
0.185
0ng/L 0.027 0.024 0
0.022

Precision

We measured random samples of E0437Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 218.9 10.17 4.6
Intra-Assay 2 18 565.9 26.59 4.7
Intra-Assay 3 18 181.9 10.54 5.8

Protocols

References (1)

Comparison of the effects of probiotic strains (Lactobacillus gasseri, Lactiplantibacillus plantarum, Lactobacillus acidophilus, and Limosilactobacillus fermentum) isolated from human and food products on the immune response of CT26 tumor-bearing mice

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  • Size

    Code

    Price

  • 48T

    E0437Mo

    $320.00

  • 96T

    E0437Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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