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Home > Product > ELISA Kit > Mouse Caspase-9 ELISA Kit

Mouse Caspase-9 ELISA Kit

Mouse Caspase-9 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.29ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 3.29ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Caspase-9

Code

E0627Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.29ng/L

Detection range

7-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) (By similarity).

UniProt accession

MASS(Da)

49,979

GeneID

12371

Synonyms

APAF-3;Apoptotic protease Mch-6;Apoptotic protease-activating factor 3;CASP 9;CASP9;CASP-9;Caspase-9;Caspase-9 subunit p10;Caspase-9 subunit p35;ICE-LAP6;ICE-like apoptotic protease 6

Gene names

CASP9

Research area

Others

Target protein

CASP9

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0627Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 2.326 2.204 2.158
2.082
400ng/L 1.37 1.385 1.338
1.399
200ng/L 0.742 0.834 0.788
0.926
100ng/L 0.466 0.487 0.441
0.508
50ng/L 0.311 0.274 0.228
0.237
0ng/L 0.043 0.046 0
0.05

Precision

We measured random samples of E0627Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 88.5 4.51 5.1
Intra-Assay 2 18 355.1 15.75 4.4
Intra-Assay 3 18 174 10.27 5.9

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E0627Mo

    $320.00

  • 96T

    E0627Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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