Full product name
Caspase 8
Code
E0789Mo
Assay type
Sandwich
Size
48T,96T
Sensitivity
9.09ng/L
Detection range
20-6000ng/L
Sample type
Serum, plasma, cell culture supernates
Species
Mouse
Storage
2-8ºC
Assay time
1h 30m
Background
Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood (PubMed: 18455983, PubMed: 30361383, PubMed: 30381$458.00|$300.00, PubMed: 31511692, PubMed: 31748744). Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death (PubMed: 9654089, PubMed: 9837723, PubMed: 24813849, PubMed: 24813850). Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10 (By similarity). Binding to the adapter molecule FADD recruits it to either receptor TNFRSF6/FAS mediated or TNFRSF1A (PubMed: 29440439). The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation (By similarity). The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases (By similarity). Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC (By similarity). In addition to extrinsic apoptosis, also acts as a negative regulator of necroptosis: acts by cleaving RIPK1 at 'Asp-325', which is crucial to inhibit RIPK1 kinase activity, limiting TNF-induced apoptosis, necroptosis and inflammatory response (PubMed: 31511692). Also able to initiate pyroptosis by mediating cleavage and activation of gasdermin-D (GSDMD): GSDMD cleavage promoting release of the N-terminal moiety (Gasdermin-D, N-terminal) that binds to membranes and forms pores, triggering pyroptosis (PubMed: 30361383, PubMed: 30381$458.00|$300.00). Initiates pyroptosis following inactivation of MAP3K7/TAK1 (PubMed: 30361383, PubMed: 30381$458.00|$300.00). Also acts as a regulator of innate immunity by mediating cleavage and inactivation of N4BP1 downstream of TLR3 or TLR4, thereby promoting cytokine production (PubMed: 32971525). May participate in the Granzyme B (GZMB) cell death pathways (By similarity). Cleaves PARP1 (By similarity).
UniProt accession
MASS（Da）
55,357
GeneID
12370
Synonyms
CASP 8;CASP8;CASP-8;Caspase-8;Caspase-8 subunit p10;Caspase-8 subunit p18
Gene names
CASP8
Research area
Cell Biology; Cancer; Metabolism
Target protein
CASP8
