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Home > Product > ELISA Kit > Mouse Secreted Frizzled Related Protein 1, SFRP1 ELISA Kit

Mouse Secreted Frizzled Related Protein 1, SFRP1 ELISA Kit

Mouse Secreted Frizzled Related Protein 1, SFRP1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.29ng/mL

Detection range: 10-2000ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 5.29ng/mL

Detection range: 10-2000ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Secreted Frizzled Related Protein 1

Code

E0902Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.29ng/mL

Detection range

10-2000ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase. In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription (By similarity).

UniProt accession

MASS(Da)

35,413

GeneID

20377

Synonyms

Secreted frizzled-related protein 1;SFRP 1;SFRP1;sFRP-1

Gene names

SFRP1

Research area

Cell Biology; Neuroscience; Stem Cells; Developmental Biology

Target protein

SFRP1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0902Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/mL 2.537 2.448 2.405
2.36
600ng/mL 1.513 1.527 1.484
1.54
300ng/mL 0.729 0.768 0.725
0.808
150ng/mL 0.458 0.426 0.383
0.394
75ng/mL 0.249 0.231 0.188
0.213
0ng/mL 0.046 0.043 0
0.04

Precision

We measured random samples of E0902Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 206 10.8 5.2
Intra-Assay 2 18 629 19.33 3.1
Intra-Assay 3 18 392.7 20.55 5.2

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0902Mo

    $320.00

  • 96T

    E0902Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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