Full product name
Glycogen Synthase Kinase-3 Alpha
Code
E1250Mo
Assay type
Sandwich
Size
48T,96T
Sensitivity
0.026ng/ml
Detection range
0.05-30ng/ml
Sample type
Serum, plasma, cell culture supernates
Species
Mouse
Storage
2-8ºC
Assay time
1h 30m
Background
Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen Synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1 (PubMed: 15791206, PubMed: 17908561). Requires primed phosphorylation of the majority of its substrates (PubMed: 22539723). Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis (PubMed: 15791206, PubMed: 17908561). Regulates glycogen metabolism in liver, but not in muscle (PubMed: 17908561). May also mediate the development of insulin resistance by regulating activation of transcription factors (By similarity). In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin (PubMed: 15791206). Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease (By similarity). May be involved in the regulation of replication in pancreatic beta-cells (By similarity). Is necessary for the establishment of neuronal polarity and axon outgrowth (PubMed: 17391670). Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation (PubMed: 16543145). Acts as a regulator of autophagy by mediating phosphorylation of KAT5/TIP60 under starvation conditions, leading to activate KAT5/TIP60 acetyltransferase activity and promote acetylation of key autophagy regulators, such as ULK1 and RUBCNL/Pacer (PubMed: 22539723). Negatively regulates extrinsic apoptotic signaling pathway via death domain receptors. Promotes the formation of an anti-apoptotic complex, made of DDX3X, BRIC2 and GSK3B, at death receptors, including TNFRSF10B. The anti-apoptotic function is most effective with weak apoptotic signals and can be overcome by stronger stimulation (By similarity).
UniProt accession
MASS（Da）
51,661
GeneID
606496
Synonyms
Glycogen Synthase kinase-3 alpha;GSK 3A;GSK-3 alpha;GSK3A;Serine/threonine-protein kinase GSK3A
Gene names
GSK3A
Research area
Others
Target protein
GSK3A
