Product overview
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Background
Interleukin 23 (IL-23) is a member of the IL-12 cytokine family and composed of two subunits, IL-12 p40 and IL-23 p19. It is produced by antigen presenting cells and has been shown to promote the production and survival of a distinct lineage of T-cells called Th17 cells. A functional receptor for IL-23 (the IL-23 receptor) has been identified and is composed of IL-12Rβ1 and IL-23R. IL-23 is expressed chiefly by the macrophages and DCs. The IL-23R is found on memory T cells, NKT cells, macrophages, DCs, and naive T cells upon activation by TGF-β and IL-6. The main biological effects of IL-23 identified initially consist of stimulation of antigen presentation by DCs, T cell differentiation to Th17 cells, and production of interferon-γ (IFN-γ). IL-23 acts also as an end-stage effector cytokine through direct action on macrophages. This kit can be used to determine relative mass values for IL-23 p40.
UniProt accession
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Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0074Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 480ng/L | 2.132 | 2.25 | 2.222 |
| 2.369 | |||
| 240ng/L | 1.282 | 1.239 | 1.21 |
| 1.195 | |||
| 120ng/L | 0.643 | 0.634 | 0.605 |
| 0.625 | |||
| 60ng/L | 0.325 | 0.37 | 0.342 |
| 0.415 | |||
| 30ng/L | 0.146 | 0.146 | 0.117 |
| 0.146 | |||
| 0ng/L | 0.027 | 0.029 | 0 |
| 0.03 |
Precision
We measured random samples of E0074Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 144.2 | 6.43 | 4.5 |
| Intra-Assay | 2 | 18 | 243.3 | 7.11 | 2.9 |
| Intra-Assay | 3 | 18 | 86.6 | 4.9 | 5.7 |
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