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Home > Product > ELISA Kit > Mouse Leukotriene A4 Hydrolase, LTA4H ELISA Kit

Mouse Leukotriene A4 Hydrolase, LTA4H ELISA Kit

Mouse Leukotriene A4 Hydrolase, LTA4H ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.027ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.027ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Leukotriene A4 Hydrolase

Code

E1583Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.027ng/ml

Detection range

0.05-15ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Bifunctional zinc metalloenzyme that comprises both epoxide hydrolase (EH) and aminopeptidase activities (By similarity). Acts as an epoxide hydrolase to catalyze the conversion of LTA4 to the proinflammatory mediator leukotriene B4 (LTB4) (PubMed: 1881903, PubMed: 9287304). Has also aminopeptidase activity, with high affinity for N-terminal arginines of various synthetic tripeptides (By similarity). In addition to its proinflammatory EH activity, may also counteract inflammation by its aminopeptidase activity, which inactivates by cleavage another neutrophil attractant, the tripeptide Pro-Gly-Pro (PGP), a bioactive fragment of collagen generated by the action of Matrix Metalloproteinase-9 (MMP9) and prolylendopeptidase (PREPL) (PubMed: 20813919). Involved also in the biosynthesis of resolvin E1 and 18S-resolvin E1 from eicosapentaenoic acid, two lipid mediators that show potent anti-inflammatory and pro-resolving actions (By similarity).

UniProt accession

MASS(Da)

69,051

GeneID

16993

Synonyms

Leukotriene A(4 hydrolase;Leukotriene A-4 hydrolase;LTA 4H;LTA-4 hydrolase;LTA4H;Tripeptide aminopeptidase LTA4H

Gene names

LTA4H

Research area

Others

Target protein

LTA4H

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1583Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8ng/ml 2.17 2.255 2.215
2.34
4ng/ml 1.301 1.398 1.358
1.495
2ng/ml 0.855 0.822 0.782
0.788
1ng/ml 0.412 0.405 0.365
0.397
0.5ng/ml 0.297 0.3 0.26
0.304
0ng/ml 0.043 0.04 0
0.037

Precision

We measured random samples of E1583Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2.6 0.15 5.8
Intra-Assay 2 18 5.7 0.21 3.6
Intra-Assay 3 18 1.7 0.11 6.1

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E1583Mo

    $320.00

  • 96T

    E1583Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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