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Home > Product > ELISA Kit > Human Tumor Necrosis Factor Αlpha, TNF-A ELISA Kit

Human Tumor Necrosis Factor Αlpha, TNF-A ELISA Kit

Human Tumor Necrosis Factor Αlpha, TNF-A ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 1.52ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 1.52ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Tumor Necrosis Factor alpha

Code

E0082Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

1.52ng/L

Detection range

3-900ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed: 23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed: 22517918, PubMed: 16829952, PubMed: 23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (PubMed: 12794819). The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

UniProt accession

MASS(Da)

25,644

GeneID

7124

Synonyms

Cachectin;C-domain 1;C-domain 2;ICD1;ICD2;Intracellular domain 1;Intracellular domain 2;N-terminal fragment;NTF;TNF A;TNF alpha;TNFA;TNF-a;TNF-alpha;Tumor necrosis factor;Tumor necrosis factor ligand superfamily member 2;Tumor necrosis factor, membrane form;Tumor necrosis factor, soluble form

Gene names

TNFA

Research area

Immunology; Signal Transduction; Cancer; Microbiology

Target protein

TNFA

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0082Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
480ng/L 1.949 1.985 1.942
2.02
240ng/L 0.914 1.026 0.983
1.138
120ng/L 0.43 0.515 0.473
0.601
60ng/L 0.293 0.277 0.234
0.261
30ng/L 0.141 0.1 0.057
0.059
0ng/L 0.054 0.043 0
0.032

Precision

We measured random samples of E0082Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 107.9 4.86 4.5
Intra-Assay 2 18 317.7 8.07 2.5
Intra-Assay 3 18 224.5 11.33 5

Protocols

References

Effect of Resveratrol on Serum Levels of Type II Collagen and Aggrecan in Patients with Knee Osteoarthritis: A Pilot Clinical Study.
Correlation between serum pro inflammatory cytokines and clinical scores of knee osteoarthritic patients using resveratrol as a supplementary therapy with meloxicam.

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Product size

Price

  • 96T

    $458.00

  • 48T

    $320.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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