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Home > Product > ELISA Kit > Mouse C-C Motif Chemokine 2, CCL2 ELISA Kit

Mouse C-C Motif Chemokine 2, CCL2 ELISA Kit

Mouse C-C Motif Chemokine 2, CCL2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.93pg/ml

Detection range: 2-600pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.93pg/ml

Detection range: 2-600pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

C-C Motif Chemokine 2

Code

E1707Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.93pg/ml

Detection range

2-600pg/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Acts as a ligand for C-C chemokine receptor CCR2 (By similarity). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (By similarity). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (PubMed: 29993042). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (PubMed: 29993042).

UniProt accession

MASS(Da)

16,326

GeneID

20296

Synonyms

C-C motif chemokine 2;CCL 2;CCL2;MCP-1;Monocyte chemoattractant protein 1;Monocyte chemotactic protein 1;Platelet-derived growth factor-inducible protein JE;Small-inducible cytokine A2

Gene names

CCL2

Research area

Immunology

Target protein

CCL2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1707Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
400pg/ml 2.35 2.294 2.269
2.239
200pg/ml 1.345 1.314 1.289
1.283
100pg/ml 0.702 0.645 0.62
0.589
50pg/ml 0.3 0.324 0.299
0.347
25pg/ml 0.195 0.204 0.178
0.212
0pg/ml 0.019 0.025 0
0.031

Precision

We measured random samples of E1707Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 293.1 10.64 3.6
Intra-Assay 2 18 85.4 4.99 5.8
Intra-Assay 3 18 172.5 8.78 5.1

Protocols

References (1)

LncRNA MEG3 up-regulates SIRT6 by ubiquitinating EZH2 and alleviates nonalcoholic fatty liver disease

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1707Mo

    $320.00

  • 96T

    E1707Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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