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Home > Product > ELISA Kit > Mouse Aromatase, ARO ELISA Kit

Mouse Aromatase, ARO ELISA Kit

Mouse Aromatase, ARO ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.48ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 1.48ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Aromatase

Code

E1861Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.48ng/L

Detection range

3-900ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively. Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid. Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen. Also displays 2-hydroxylase activity toward estrone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).

UniProt accession

MASS(Da)

58,015

GeneID

13075

Synonyms

Aromatase;CYP19A1;CYPXIX;Cytochrome P450 19A1;Cytochrome P-450AROM;Estrogen Synthase

Gene names

CYP19A1

Research area

Metabolism

Target protein

CYP19A1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1861Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
480ng/L 1.842 1.982 1.947
2.123
240ng/L 0.91 0.946 0.91
0.982
120ng/L 0.482 0.464 0.428
0.446
60ng/L 0.199 0.203 0.167
0.207
30ng/L 0.11 0.135 0.1
0.16
0ng/L 0.033 0.036 0
0.039

Precision

We measured random samples of E1861Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 95.9 5.38 5.6
Intra-Assay 2 18 333.5 8.72 2.6
Intra-Assay 3 18 231.5 6.48 2.8

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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