Product overview
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Background
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.
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Target protein
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard solution | 0.5ml x1 |
Standard diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop solution | 6ml x1 |
Substrate solution A | 6ml x1 |
Substrate solution B | 6ml x1 |
Wash buffer concentrate (25x) | 20ml x1 |
Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2452Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
800ng/L | 2.464 | 2.478 | 2.353 |
2.492 | |||
400ng/L | 1.742 | 1.776 | 1.651 |
1.81 | |||
200ng/L | 1.339 | 1.379 | 1.253 |
1.418 | |||
100ng/L | 0.914 | 0.879 | 0.754 |
0.844 | |||
50ng/L | 0.387 | 0.375 | 0.25 |
0.364 | |||
0ng/L | 0.121 | 0.125 | 0 |
0.129 |
Precision
We measured random samples of E2452Ra within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-Assay | 1 | 18 | 228.7 | 8.9 | 3.9 |
Intra-Assay | 2 | 18 | 150.6 | 11.2 | 7.4 |
Intra-Assay | 3 | 18 | 65.7 | 3.6 | 5.5 |
Protocols
References (1)
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