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Home > Product > ELISA Kit > Rat Phospho-AKT(S473) ELISA Kit

Rat Phospho-AKT(S473) ELISA Kit

Rat Phospho-AKT(S473) ELISA Kit

Product Summary

Size: 96T, 48T

Sensitivity: 4.06ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 96T, 48T

Sensitivity: 4.06ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Phospho-AKT(S473)

Code

E2452Ra

Assay type

Sandwich

Size

96T, 48T

Sensitivity

4.06ng/L

Detection range

7-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.

UniProt accession

MASS(Da)

55735

GeneID

24185

Synonyms

RAC-alpha serine/threonine-protein kinase

Gene names

AKT

Research area

Others

Target protein

AKT

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2452Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 2.464 2.478 2.353
2.492
400ng/L 1.742 1.776 1.651
1.81
200ng/L 1.339 1.379 1.253
1.418
100ng/L 0.914 0.879 0.754
0.844
50ng/L 0.387 0.375 0.25
0.364
0ng/L 0.121 0.125 0
0.129

Precision

We measured random samples of E2452Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 228.7 8.9 3.9
Intra-Assay 2 18 150.6 11.2 7.4
Intra-Assay 3 18 65.7 3.6 5.5

Protocols

References (1)

Mitoquinone Alleviates Donation after Cardiac Death Kidney Injury during Hypothermic Machine Perfusion in Rat Model

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  • Size

    Code

    Price

  • 96T

    E2452Ra

    $458.00

  • 48T

    E2452Ra

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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