Product overview
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Assay type
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Background
Metallothioneins (MTs) are a class of ubiquitous, low molecular weight, cysteine-rich, metal binding proteins that function in the metabolism of zinc and copper. MT protects against exposure to toxic metals such as mercury and cadmium as well as a variety of other stress conditions. There are four mammalian MT isoforms: MT-I, -II, -III, -IV, each of which is encoded by individual genes. MT expression is induced by heavy metal cations, starvation, elevated temperature, lipopolysaccharide, inflammatory cytokines, free radicals, and ionizing radiation.
UniProt accession
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Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1733Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 24ng/ml | 2.169 | 2.115 | 2.056 |
| 2.061 | |||
| 12ng/ml | 1.727 | 1.613 | 1.554 |
| 1.499 | |||
| 6ng/ml | 1.147 | 1.142 | 1.082 |
| 1.136 | |||
| 3ng/ml | 0.629 | 0.643 | 0.584 |
| 0.658 | |||
| 1.5ng/ml | 0.472 | 0.445 | 0.386 |
| 0.418 | |||
| 0ng/ml | 0.057 | 0.059 | 0 |
| 0.061 |
Precision
We measured random samples of E1733Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 2.7 | 0.16 | 6 |
| Intra-Assay | 2 | 18 | 16.1 | 1.11 | 6.9 |
| Intra-Assay | 3 | 18 | 3.7 | 0.23 | 6.1 |
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