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Home > Product > ELISA Kit > Mouse Dedicator of Cytokinesis Protein 1, DOCK1 ELISA Kit

Mouse Dedicator of Cytokinesis Protein 1, DOCK1 ELISA Kit

Mouse Dedicator of Cytokinesis Protein 1, DOCK1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 14.13ng/L

Detection range: 30-9000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 14.13ng/L

Detection range: 30-9000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Dedicator of Cytokinesis Protein 1

Code

E1998Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

14.13ng/L

Detection range

30-9000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Along with DOCK1, mediates CRK/CRKL regulation of epithelial and endothelial cell spreading and migration on type IV collagen. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.

UniProt accession

MASS(Da)

215,085

GeneID

330662

Synonyms

180 kDa protein downstream of CRK;Dedicator of cytokinesis protein 1;DOCK 1;DOCK1;DOCK180

Gene names

DOCK1

Research area

Others

Target protein

DOCK1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1998Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4800ng/L 2.069 2.082 2.037
2.095
2400ng/L 1.54 1.594 1.55
1.648
1200ng/L 1.004 1.07 1.025
1.135
600ng/L 0.676 0.695 0.651
0.715
300ng/L 0.462 0.461 0.416
0.459
0ng/L 0.06 0.045 0
0.029

Precision

We measured random samples of E1998Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1063.9 68.75 6.5
Intra-Assay 2 18 2025.8 114.18 5.6
Intra-Assay 3 18 481.7 35.31 7.3

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

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Lead time: Within one week

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