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Home > Product > ELISA Kit > Mouse Dihydrofolate Reductase, DHFR ELISA Kit

Mouse Dihydrofolate Reductase, DHFR ELISA Kit

Mouse Dihydrofolate Reductase, DHFR ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.42ng/L

Detection range: 20-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.42ng/L

Detection range: 20-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Dihydrofolate Reductase

Code

E2008Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.42ng/L

Detection range

20-3500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway (PubMed: 25980602). Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (PubMed: 25980602). Binds its own mRNA.

UniProt accession

MASS(Da)

21,606

GeneID

13361

Synonyms

DHFR;Dihydrofolate reductase

Gene names

DHFR

Research area

Signal Transduction

Target protein

DHFR

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2008Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 2.106 2.125 2.079
2.144
1000ng/L 1.516 1.457 1.411
1.398
500ng/L 0.559 0.656 0.61
0.754
250ng/L 0.433 0.386 0.34
0.338
125ng/L 0.126 0.132 0.086
0.138
0ng/L 0.053 0.046 0
0.039

Precision

We measured random samples of E2008Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 593.9 23.52 4
Intra-Assay 2 18 1324.6 43.95 3.3
Intra-Assay 3 18 278.5 11.06 4

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2008Mo

    $320.00

  • 96T

    E2008Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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