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Home > Product > ELISA Kit > Mouse Leucine-rich Repeat-containing G-protein Coupled Receptor 5, LGR5 ELISA Kit

Mouse Leucine-rich Repeat-containing G-protein Coupled Receptor 5, LGR5 ELISA Kit

Mouse Leucine-rich Repeat-containing G-protein Coupled Receptor 5, LGR5 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.93ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.93ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Leucine-Rich Repeat-Containing G-Protein Coupled Receptor 5

Code

E2015Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.93ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.

UniProt accession

MASS(Da)

99,666

GeneID

14160

Synonyms

G-protein coupled receptor 49;Leucine-rich repeat-containing G-protein coupled receptor 5;LGR 5;LGR5;Orphan G-protein coupled receptor FEX

Gene names

LGR5

Research area

Signal Transduction

Target protein

LGR5

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2015Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.268 2.301 2.253
2.333
1200ng/L 1.927 1.899 1.851
1.871
600ng/L 1.244 1.164 1.115
1.084
300ng/L 0.685 0.683 0.635
0.681
150ng/L 0.377 0.362 0.314
0.348
0ng/L 0.04 0.048 0
0.057

Precision

We measured random samples of E2015Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 674.6 22.5 3.3
Intra-Assay 2 18 578.1 33.58 5.8
Intra-Assay 3 18 215.9 9.5 4.4

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2015Mo

    $320.00

  • 96T

    E2015Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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