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Home > Product > ELISA Kit > Mouse Polycomb Complex Protein Bmi-1, BMI-1 ELISA Kit

Mouse Polycomb Complex Protein Bmi-1, BMI-1 ELISA Kit

Mouse Polycomb Complex Protein Bmi-1, BMI-1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.022ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.022ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Polycomb complex protein BMI-1

Code

E2018Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.022ng/ml

Detection range

0.05-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A. In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2 (By similarity).

UniProt accession

MASS(Da)

36,708

GeneID

12151

Synonyms

BMI 1;BMI1;Polycomb complex protein BMI-1;Polycomb group RING finger protein 4

Gene names

BMI1

Research area

Cell Biology; Epigenetics and Nuclear Signaling; Stem Cells

Target protein

BMI1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2018Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/ml 2.307 2.183 2.129
2.058
8ng/ml 1.63 1.586 1.532
1.541
4ng/ml 1.026 1.007 0.954
0.989
2ng/ml 0.722 0.724 0.67
0.725
1ng/ml 0.496 0.446 0.393
0.397
0ng/ml 0.062 0.054 0
0.045

Precision

We measured random samples of E2018Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 7.43 0.33 4.41
Intra-Assay 2 18 3.63 0.21 5.76
Intra-Assay 3 18 1.99 0.11 5.55

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2018Mo

    $320.00

  • 96T

    E2018Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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