Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
UniProt accession
MASS（Da）
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 wells * 8 detachable strips |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0034Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 32ng/ml | 1.981 | 1.904 | 1.846 |
| 1.828 | |||
| 16ng/ml | 0.919 | 0.982 | 0.924 |
| 1.045 | |||
| 8ng/ml | 0.434 | 0.525 | 0.467 |
| 0.616 | |||
| 4ng/ml | 0.314 | 0.284 | 0.226 |
| 0.255 | |||
| 2ng/ml | 0.102 | 0.098 | 0.04 |
| 0.094 | |||
| 0ng/ml | 0.057 | 0.058 | 0 |
| 0.059 |
Precision
We measured random samples of E0034Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 11.2 | 0.6 | 5.4 |
| Intra-assay | 2 | 18 | 14.2 | 0.6 | 4.4 |
| Intra-assay | 3 | 18 | 27.3 | 0.9 | 3.3 |
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