Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
UniProt accession
MASS(Da)
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 wells * 8 detachable strips |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0725Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 12ng/ml | 1.988 | 1.936 | 1.892 |
| 1.885 | |||
| 6ng/ml | 0.984 | 0.95 | 0.906 |
| 0.917 | |||
| 3ng/ml | 0.395 | 0.402 | 0.357 |
| 0.409 | |||
| 1.5ng/ml | 0.165 | 0.181 | 0.136 |
| 0.197 | |||
| 0.75ng/ml | 0.119 | 0.124 | 0.08 |
| 0.13 | |||
| 0ng/ml | 0.034 | 0.045 | 0 |
| 0.056 |
Precision
We measured random samples of E0725Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 10.6 | 0.3 | 2.6 |
| Intra-assay | 2 | 18 | 4.3 | 0.2 | 3.7 |
| Intra-assay | 3 | 18 | 5.2 | 0.2 | 3.6 |
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