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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0003Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64ng/ml	2.367	2.267	2.206
	2.167
32ng/ml	1.737	1.775	1.714
	1.813
16ng/ml	1.305	1.201	1.139
	1.097
8ng/ml	0.68	0.648	0.587
	0.616
4ng/ml	0.374	0.364	0.303
	0.355
0ng/ml	0.061	0.061	0
	0.061

We measured random samples of E0003Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	10.1	0.5	4.8
Intra-assay	2	18	25.9	1.2	4.6
Intra-assay	3	18	5.9	0.4	6.1

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