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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0918Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480U/L	2.031	2.162	2.117
	2.293
240U/L	1.619	1.555	1.509
	1.491
120U/L	1.075	1.071	1.025
	1.067
60U/L	0.642	0.665	0.619
	0.687
30U/L	0.439	0.467	0.421
	0.494
0U/L	0.037	0.046	0
	0.054

We measured random samples of E0918Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	28.7	2.1	7.3
Intra-assay	2	18	310.6	17.2	5.5
Intra-assay	3	18	97.6	7.6	7.8

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