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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0164Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
160ng/ml	2.326	2.26	2.206
	2.195
80ng/ml	1.407	1.355	1.301
	1.304
40ng/ml	0.735	0.842	0.788
	0.949
20ng/ml	0.472	0.454	0.4
	0.436
10ng/ml	0.241	0.248	0.194
	0.256
0ng/ml	0.059	0.054	0
	0.049

We measured random samples of E0164Rb within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	101.8	3.8	3.7
Intra-assay	2	18	24.9	1.7	7
Intra-assay	3	18	49.1	2.2	4.5

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