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Home > Product > ELISA Kit > Mouse Major Centromere Autoantigen B, CENPB ELISA Kit

Mouse Major Centromere Autoantigen B, CENPB ELISA Kit

Mouse Major Centromere Autoantigen B, CENPB ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.45ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 7.45ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Major Centromere Autoantigen B

Code

E2232Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.45ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Interacts with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.

UniProt accession

MASS(Da)

65,381

GeneID

12616

Synonyms

CENPB;CENP-B;Centromere protein B;Major centromere autoantigen B

Gene names

CENPB

Research area

Epigenetics and Nuclear Signaling

Target protein

CENPB

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2232Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 1.978 1.968 1.929
1.958
800ng/L 0.865 0.914 0.875
0.962
400ng/L 0.481 0.449 0.41
0.416
200ng/L 0.283 0.283 0.244
0.283
100ng/L 0.148 0.15 0.111
0.152
0ng/L 0.039 0.039 0
0.04

Precision

We measured random samples of E2232Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 488.6 27.34 5.6
Intra-Assay 2 18 1089.3 25.59 2.3
Intra-Assay 3 18 837.4 37.45 4.5

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2232Mo

    $320.00

  • 96T

    E2232Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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