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Home > Product > ELISA Kit > Mouse Neudesin, NENF ELISA Kit

Mouse Neudesin, NENF ELISA Kit

Mouse Neudesin, NENF ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.53ng/L

Detection range: 1-400ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.53ng/L

Detection range: 1-400ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Neudesin

Code

E2288Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.53ng/L

Detection range

1-400ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Acts as a neurotrophic factor in postnatal mature neurons, enhancing neuronal survival (PubMed: 15605373, PubMed: 31536960). Promotes cell proliferation and neurogenesis in undifferentiated neural pro-genitor cells at the embryonic stage and inhibits differentiation of astrocytes (PubMed: 16547973). Its neurotrophic activity is exerted via MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT pathways (PubMed: 15605373, PubMed: 16547973). Neurotrophic activity is enhanced by binding to heme (PubMed: 18056703). Acts also as an anorexigenic neurotrophic factor that contributes to energy balance (PubMed: 23576617).

UniProt accession

MASS(Da)

18,904

GeneID

66208

Synonyms

NENF;Neudesin;Neuron-derived neurotrophic factor;Secreted protein of unknown function;SPUF protein

Gene names

NENF

Research area

Others

Target protein

NENF

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2288Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
240ng/L 2.162 2.126 2.078
2.09
120ng/L 1.078 1.022 0.974
0.966
60ng/L 0.443 0.45 0.403
0.457
30ng/L 0.192 0.252 0.205
0.312
15ng/L 0.099 0.086 0.038
0.073
0ng/L 0.037 0.048 0
0.059

Precision

We measured random samples of E2288Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 66.5 2.95 4.4
Intra-Assay 2 18 123.6 5.23 4.2
Intra-Assay 3 18 175 3.53 2

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2288Mo

    $320.00

  • 96T

    E2288Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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