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Home > Product > ELISA Kit > Mouse NF-kappa-B Inhibitor Alpha, NFKBIA ELISA Kit

Mouse NF-kappa-B Inhibitor Alpha, NFKBIA ELISA Kit

Mouse NF-kappa-B Inhibitor Alpha, NFKBIA ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.93ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.93ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Nf-Kappa-B Inhibitor Alpha

Code

E2305Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.93ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

UniProt accession

MASS(Da)

35,071

GeneID

18035

Synonyms

IkappaBalpha;I-kappa-B-alpha;IkB-alpha;NF-kappa-B inhibitor alpha;NFKBIA

Gene names

NFKBIA

Research area

Others

Target protein

NFKBIA

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2305Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.149 2.019 1.989
1.889
1200ng/L 0.947 1.019 0.988
1.09
600ng/L 0.49 0.537 0.507
0.584
300ng/L 0.187 0.239 0.209
0.291
150ng/L 0.109 0.132 0.102
0.156
0ng/L 0.032 0.03 0
0.028

Precision

We measured random samples of E2305Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1680.5 46.96 2.8
Intra-Assay 2 18 438.8 23.67 5.4
Intra-Assay 3 18 1160.7 55.54 4.8

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2305Mo

    $320.00

  • 96T

    E2305Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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