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Home > Product > ELISA Kit > Mouse Palmitoyl-protein Thioesterase 1, PPT1 ELISA Kit

Mouse Palmitoyl-protein Thioesterase 1, PPT1 ELISA Kit

Mouse Palmitoyl-protein Thioesterase 1, PPT1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.62ng/L

Detection range: 20-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.62ng/L

Detection range: 20-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Palmitoyl-Protein Thioesterase 1

Code

E2325Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.62ng/L

Detection range

20-3500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or Peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.

UniProt accession

MASS(Da)

34,490

GeneID

19063

Synonyms

Palmitoyl-protein hydrolase 1;Palmitoyl-protein thioesterase 1;PPT 1;PPT1;PPT-1

Gene names

PPT1

Research area

Others

Target protein

PPT1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2325Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 2.62 2.558 2.499
2.495
1000ng/L 1.511 1.465 1.407
1.419
500ng/L 0.713 0.788 0.73
0.863
250ng/L 0.498 0.468 0.409
0.437
125ng/L 0.225 0.245 0.186
0.264
0ng/L 0.053 0.058 0
0.064

Precision

We measured random samples of E2325Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 978.1 31.86 3.3
Intra-Assay 2 18 844.2 42.43 5
Intra-Assay 3 18 277.6 12.71 4.6

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2325Mo

    $320.00

  • 96T

    E2325Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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