Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility.
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2331Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 24ng/mL | 2.031 | 2.086 | 2.047 |
| 2.14 | |||
| 12ng/mL | 1.373 | 1.354 | 1.315 |
| 1.335 | |||
| 6ng/mL | 0.551 | 0.55 | 0.511 |
| 0.549 | |||
| 3ng/mL | 0.307 | 0.358 | 0.319 |
| 0.41 | |||
| 1.5ng/mL | 0.208 | 0.182 | 0.143 |
| 0.156 | |||
| 0ng/mL | 0.023 | 0.039 | 0 |
| 0.056 |
Precision
We measured random samples of E2331Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 4.2 | 0.2 | 4.6 |
| Intra-Assay | 2 | 18 | 10.3 | 0.41 | 4 |
| Intra-Assay | 3 | 18 | 17.8 | 0.77 | 4.3 |
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