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Home > Product > ELISA Kit > Mouse R-spondin-1, RSPO1 ELISA Kit

Mouse R-spondin-1, RSPO1 ELISA Kit

Mouse R-spondin-1, RSPO1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.98ng/L

Detection range: 2-700ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.98ng/L

Detection range: 2-700ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

R-Spondin-1

Code

E2434Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.98ng/L

Detection range

2-700ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes (PubMed: 21693646). Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination (By similarity). Regulates Wnt signaling by antagonizing DKK1/KREM1-mediated internalization of LRP6 through an interaction with KREM1 (By similarity).

UniProt accession

MASS(Da)

29,332

GeneID

192199

Synonyms

Cristin-3;Cysteine-rich and single thrombospondin domain-containing protein 3;mCristin-3;Roof plate-specific spondin-1;RSPO 1;RSPO1;R-spondin-1

Gene names

RSPO1

Research area

Developmental Biology

Target protein

RSPO1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2434Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
400ng/L 1.714 1.791 1.74
1.867
200ng/L 1.136 1.007 0.956
0.877
100ng/L 0.46 0.541 0.49
0.623
50ng/L 0.138 0.164 0.113
0.19
25ng/L 0.055 0.098 0.047
0.142
0ng/L 0.041 0.051 0
0.061

Precision

We measured random samples of E2434Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 248 7.17 2.9
Intra-Assay 2 18 176.1 7.96 4.5
Intra-Assay 3 18 139.1 7.22 5.2

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2434Mo

    $320.00

  • 96T

    E2434Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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