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Home > Product > ELISA Kit > Mouse Runt-related Transcription Factor 2, RUNX2 ELISA Kit

Mouse Runt-related Transcription Factor 2, RUNX2 ELISA Kit

Mouse Runt-related Transcription Factor 2, RUNX2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.526ng/mL

Detection range: 1-400ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.526ng/mL

Detection range: 1-400ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Runt-Related Transcription Factor 2

Code

E2438Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.526ng/mL

Detection range

1-400ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. Inhibits KAT6B-dependent transcriptional activation (By similarity). In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).

UniProt accession

MASS(Da)

66,205

GeneID

12393

Synonyms

Acute myeloid leukemia 3 protein;CBF-alpha-1;Core-binding factor subunit alpha-1;Oncogene AML-3;OSF-2;Osteoblast-specific transcription factor 2;PEA2-alpha A;PEBP2-alpha A;Polyomavirus enhancer-binding protein 2 alpha A subunit;Runt-related transcription factor 2;RUNX 2;RUNX2;SL3/AKV core-binding factor alpha A subunit;SL3-3 enhancer factor 1 alpha A subunit

Gene names

RUNX2

Research area

Others

Target protein

RUNX2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2438Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
240ng/mL 2.288 2.318 2.262
2.349
120ng/mL 1.308 1.379 1.323
1.45
60ng/mL 0.849 0.769 0.714
0.69
30ng/mL 0.419 0.453 0.397
0.487
15ng/mL 0.315 0.318 0.263
0.321
0ng/mL 0.048 0.056 0
0.064

Precision

We measured random samples of E2438Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 41.3 2.95 7.1
Intra-Assay 2 18 125.7 3.96 3.1
Intra-Assay 3 18 77.7 4.02 5.2

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2438Mo

    $320.00

  • 96T

    E2438Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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