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Home > Product > ELISA Kit > Mouse Serum Response Factor, SRF ELISA Kit

Mouse Serum Response Factor, SRF ELISA Kit

Mouse Serum Response Factor, SRF ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.26ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 5.26ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Serum Response Factor

Code

E2466Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.26ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS) (PubMed: 24732378). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration (PubMed: 12732141, PubMed: 19350017, PubMed: 24732378). The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics (PubMed: 24732378). Required for cardiac differentiation and maturation (PubMed: 15169892).

UniProt accession

MASS(Da)

51,247

GeneID

20807

Synonyms

CCK4;PTK7;Serum response factor;SRF

Gene names

SRF

Research area

Tags & Cell Markers

Target protein

SRF

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2466Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.083 2.146 2.099
2.209
600ng/L 1.372 1.348 1.302
1.325
300ng/L 0.702 0.78 0.733
0.858
150ng/L 0.396 0.456 0.409
0.516
75ng/L 0.168 0.197 0.15
0.225
0ng/L 0.037 0.047 0
0.056

Precision

We measured random samples of E2466Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 665.2 24.87 3.7
Intra-Assay 2 18 147.5 9.67 6.6
Intra-Assay 3 18 456.4 22.17 4.9

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2466Mo

    $320.00

  • 96T

    E2466Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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