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Home > Product > ELISA Kit > Mouse Tyrosine-protein Phosphatase Non-receptor Type 4, PTPN4 ELISA Kit

Mouse Tyrosine-protein Phosphatase Non-receptor Type 4, PTPN4 ELISA Kit

Mouse Tyrosine-protein Phosphatase Non-receptor Type 4, PTPN4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.27ng/L

Detection range: 2-600ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 1.27ng/L

Detection range: 2-600ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Tyrosine-Protein Phosphatase Non-Receptor Type 4

Code

E2548Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.27ng/L

Detection range

2-600ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Phosphatase that plays a role in immunity, learning, synaptic plasticity or cell homeostasis (PubMed: 17953619, PubMed: 25825441). Regulates neuronal cell homeostasis by protecting neurons against apoptosis (By similarity). Negatively regulates TLR4-induced interferon beta production by dephosphorylating adapter TICAM2 and inhibiting subsequent TRAM-TRIF interaction (PubMed: 25825441). Dephosphorylates also the immunoreceptor tyrosine-based activation motifs/ITAMs of the TCR zeta subunit and thereby negatively regulates TCR-mediated signaling pathway (PubMed: 18614237). May act at junctions between the membrane and the cytoskeleton.

UniProt accession

MASS(Da)

105,832

GeneID

19258

Synonyms

PTPN 4;PTPN4;Testis-enriched protein tyrosine phosphatase;Tyrosine-protein phosphatase non-receptor type 4

Gene names

PTPN4

Research area

Others

Target protein

PTPN4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2548Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
320ng/L 2.072 2.171 2.138
2.271
160ng/L 1.145 1.221 1.187
1.296
80ng/L 0.706 0.759 0.726
0.812
40ng/L 0.448 0.423 0.39
0.399
20ng/L 0.19 0.215 0.182
0.24
0ng/L 0.024 0.033 0
0.042

Precision

We measured random samples of E2548Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 106.7 6.35 5.9
Intra-Assay 2 18 241.7 7.99 3.3
Intra-Assay 3 18 41.4 2.23 5.4

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2548Mo

    $320.00

  • 96T

    E2548Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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