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Home > Product > ELISA Kit > Mouse Ceramide Kinase, CERK ELISA Kit

Mouse Ceramide Kinase, CERK ELISA Kit

Mouse Ceramide Kinase, CERK ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.014ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.014ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Ceramide Kinase

Code

E2582Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.014ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate (PubMed: 16269826). Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines (PubMed: 16269826). Shows a greater preference for D-erythro isomer of ceramides (By similarity). Binds phosphoinositides (By similarity).

UniProt accession

MASS(Da)

59,843

GeneID

223753

Synonyms

Acylsphingosine kinase;Ceramide kinase;CERK;mCERK

Gene names

CERK

Research area

Cell Biology

Target protein

CERK

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2582Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.365 2.209 2.15
2.052
6ng/ml 1.145 1.201 1.143
1.257
3ng/ml 0.666 0.704 0.645
0.742
1.5ng/ml 0.465 0.478 0.42
0.492
0.75ng/ml 0.234 0.215 0.157
0.196
0ng/ml 0.053 0.058 0
0.063

Precision

We measured random samples of E2582Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4 0.26 6.4
Intra-Assay 2 18 7.5 0.2 2.7
Intra-Assay 3 18 2.5 0.13 5.4

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2582Mo

    $320.00

  • 96T

    E2582Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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