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Home > Product > ELISA Kit > Mouse Nad-dependent Protein Deacetylase Sirtuin-3, SIRT3 ELISA Kit

Mouse Nad-dependent Protein Deacetylase Sirtuin-3, SIRT3 ELISA Kit

Mouse Nad-dependent Protein Deacetylase Sirtuin-3, SIRT3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.047ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.047ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Nad-Dependent Protein Deacetylase Sirtuin-3

Code

E2586Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.047ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

NAD-dependent protein deacetylase (PubMed: 23835326, PubMed: 17923681, PubMed: 18794531, PubMed: 21172655, PubMed: 26620563). Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues (PubMed: 23835326, PubMed: 17923681, PubMed: 18794531, PubMed: 21172655). Known targets include ACSS1, IDH, GDH, PDHA1, SOD2, LCAD, SDHA and the ATP Synthase subunit ATP5PO (PubMed: 16790548, PubMed: 18794531, PubMed: 21172655). Contributes to the regulation of the cellular energy metabolism (PubMed: 23835326). Important for regulating tissue-specific ATP levels (PubMed: 18794531, PubMed: 24252090). In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription (PubMed: 23283301). Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide Synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation (PubMed: 26620563).

UniProt accession

MASS(Da)

36,615

GeneID

64384

Synonyms

mSIR2L3;NAD-dependent protein deacetylase sirtuin-3;Regulatory protein SIR2 homolog 3;SIR2-like protein 3;SIRT 3;SIRT3

Gene names

SIRT3

Research area

Others

Target protein

SIRT3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2586Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.078 2.187 2.139
2.295
12ng/ml 1.193 1.257 1.209
1.321
6ng/ml 0.822 0.795 0.747
0.768
3ng/ml 0.506 0.504 0.456
0.502
1.5ng/ml 0.227 0.242 0.194
0.256
0ng/ml 0.037 0.048 0
0.058

Precision

We measured random samples of E2586Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.7 0.34 6
Intra-Assay 2 18 14.4 0.53 3.7
Intra-Assay 3 18 8.7 0.52 6

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2586Mo

    $320.00

  • 96T

    E2586Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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