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Home > Product > ELISA Kit > Human Interleukin 17, IL-17 ELISA Kit

Human Interleukin 17, IL-17 ELISA Kit

Human Interleukin 17, IL-17 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 1.06ng/L

Detection range: 2-600ng/L

Sample type: Serum, plasma, cell culture supernates, ascites, tissue homogenates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 1.06ng/L

Detection range: 2-600ng/L

Sample type: Serum, plasma, cell culture supernates, ascites, tissue homogenates

Reactive with: Human

Product overview

Full product name

Interleukin 17

Code

E0142Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

1.06ng/L

Detection range

2-600ng/L

Sample type

Serum, plasma, cell culture supernates, ascites, tissue homogenates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity (PubMed: 24120361). Signals via IL17RA-IL17RC heterodimeric receptor complex, triggering homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter. This leads to downstream TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides and matrix metalloproteinases, with potential strong immune inflammation (PubMed: 19825828, PubMed: 21350122, PubMed: 17911633, PubMed: 18684971, PubMed: 8676080, PubMed: 24120361). Plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. As a signature effector cytokine of T-helper 17 cells (Th17), primarily induces neutrophil activation and recruitment at infection and inflammatory sites (By similarity). In airway epithelium, mediates neutrophil chemotaxis via induction of CXCL1 and CXCL5 chemokines (By similarity). In secondary lymphoid organs, contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells (By similarity). Effector cytokine of a subset of gamma-delta T cells that functions as part of an inflammatory circuit downstream IL1B, TLR2 and IL23A-IL12B to promote neutrophil recruitment for efficient bacterial clearance (By similarity). Effector cytokine of innate immune cells including invariant natural killer cell (iNKT) and group 3 innate lymphoid cells that mediate initial neutrophilic inflammation (By similarity). Involved in the maintenance of the integrity of epithelial barriers during homeostasis and pathogen infection (PubMed: 21350122). Upon acute injury, has a direct role in epithelial barrier formation by regulating OCLN localization and tight junction biogenesis (By similarity). As part of the mucosal immune response induced by commensal bacteria, enhances host's ability to resist pathogenic bacterial and fungal infections by promoting neutrophil recruitment and antimicrobial peptides release (By similarity). In synergy with IL17F, mediates the production of antimicrobial beta-defensins DEFB1, DEFB103A, and DEFB104A by mucosal epithelial cells, limiting the entry of microbes through the epithelial barriers (By similarity). Involved in antiviral host defense through various mechanisms (By similarity). Enhances immunity against West Nile virus by promoting T cell cytotoxicity (By similarity). May play a beneficial role in influenza A virus (H5N1) infection by enhancing B cell recruitment and immune response in the lung (By similarity). Contributes to influenza A virus (H1N1) clearance by driving the differentiation of B-1a B cells, providing for production of virus-specific IgM antibodies at first line of host defense (By similarity).

UniProt accession

MASS(Da)

17,504

GeneID

3605

Synonyms

Interleukin-17A; IL-17; IL17A; IL 17A; IL-17A; Cytotoxic T-lymphocyte-associated antigen 8; CTLA-8

Gene names

IL17A

Research area

Immunology

Target protein

IL17A

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Human Interleukin 17, IL-17 ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
320ng/L 2.374 2.318 2.272
2.261
160ng/L 1.384 1.263 1.218
1.143
80ng/L 0.692 0.705 0.659
0.718
40ng/L 0.391 0.412 0.366
0.432
20ng/L 0.255 0.229 0.184
0.204
0ng/L 0.059 0.046 0
0.033

Precision

We measured random samples of E0142Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 62.3 3.09 5
Intra-Assay 2 18 166.9 5.37 3.2
Intra-Assay 3 18 90.9 4.87 5.4

Protocols

References

Are patients with vitiligo more prone to myocardial infarction?: a case-control study

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Product size

Price

  • 96T

    $458.00

  • 48T

    $300.00

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Lead time: Within one week

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