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Home > Product > ELISA Kit > Mouse Stromal Cell-derived Factor 1, CXCL12 ELISA Kit

Mouse Stromal Cell-derived Factor 1, CXCL12 ELISA Kit

Mouse Stromal Cell-derived Factor 1, CXCL12 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.11ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 5.11ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Stromal Cell-Derived Factor 1

Code

E2620Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.11ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Chemoattractant active on T-lymphocytes and monocytes but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 in a CXCR4-independent manner (By similarity). Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells (By similarity). Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell-dependent pre-B-cells (PubMed: 8134392).

UniProt accession

MASS(Da)

10,561

GeneID

20315

Synonyms

12-O-tetradecanoylphorbol 13-acetate repressed protein 1;C-X-C motif chemokine 12;CXCL 12;CXCL12;PBSF;Pre-B cell growth-stimulating factor;SDF-1;Stromal cell-derived factor 1;Thymic lymphoma cell-stimulating factor;TLSF;TPAR1

Gene names

CXCL12

Research area

Immunology; Microbiology

Target protein

CXCL12

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2620Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.045 2.083 2.032
2.122
600ng/L 1.309 1.352 1.301
1.395
300ng/L 0.768 0.776 0.724
0.783
150ng/L 0.46 0.454 0.402
0.448
75ng/L 0.253 0.261 0.209
0.269
0ng/L 0.045 0.052 0
0.058

Precision

We measured random samples of E2620Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 155.5 11.14 7.2
Intra-Assay 2 18 317.9 17.46 5.5
Intra-Assay 3 18 735.4 31.05 4.2

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2620Mo

    $320.00

  • 96T

    E2620Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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