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Home > Product > ELISA Kit > Mouse Autophagy Related Protein 4B, ATG4B ELISA Kit

Mouse Autophagy Related Protein 4B, ATG4B ELISA Kit

Mouse Autophagy Related Protein 4B, ATG4B ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Autophagy Related Protein 4B

Code

E2636Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.024ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms (By similarity).

UniProt accession

MASS(Da)

44,375

GeneID

66615

Synonyms

ATG 4B;ATG4B;AUT-like 1 cysteine endopeptidase;Autophagin-1;Autophagy-related cysteine endopeptidase 1;Autophagy-related protein 4 homolog B;Cysteine protease ATG4B

Gene names

ATG4B

Research area

Cancer

Target protein

ATG4B

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2636Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.16 2.19 2.166
2.221
6ng/ml 1.233 1.31 1.287
1.387
3ng/ml 0.567 0.586 0.563
0.605
1.5ng/ml 0.382 0.372 0.349
0.362
0.75ng/ml 0.151 0.134 0.11
0.117
0ng/ml 0.017 0.024 0
0.031

Precision

We measured random samples of E2636Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 7.4 0.2 2.7
Intra-Assay 2 18 4.4 0.19 4.2
Intra-Assay 3 18 2.3 0.1 4.3

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2636Mo

    $320.00

  • 96T

    E2636Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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