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Home > Product > ELISA Kit > Mouse Autophagy Related Protein 4C, ATG4C ELISA Kit

Mouse Autophagy Related Protein 4C, ATG4C ELISA Kit

Mouse Autophagy Related Protein 4C, ATG4C ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 14.02ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 14.02ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Autophagy Related Protein 4C

Code

E2638Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

14.02ng/L

Detection range

20-6000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms (By similarity).

UniProt accession

MASS(Da)

52,056

GeneID

242557

Synonyms

ATG 4C;ATG4C;AUT-like 3 cysteine endopeptidase;Autophagin-3;Autophagy-related cysteine endopeptidase 3;Autophagy-related protein 4 homolog C;Cysteine protease ATG4C

Gene names

ATG4C

Research area

Others

Target protein

ATG4C

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2638Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3200ng/L 2.348 2.343 2.305
2.338
1600ng/L 1.382 1.37 1.331
1.357
800ng/L 0.811 0.855 0.817
0.9
400ng/L 0.392 0.42 0.382
0.448
200ng/L 0.238 0.221 0.183
0.204
0ng/L 0.032 0.038 0
0.044

Precision

We measured random samples of E2638Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1828.3 69.67 3.8
Intra-Assay 2 18 981.6 51.75 5.3
Intra-Assay 3 18 690 42.77 6.2

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2638Mo

    $320.00

  • 96T

    E2638Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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