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Home > Product > ELISA Kit > Mouse Growth Factor Receptor-Bound Protein 2, GRB2 ELISA Kit

Mouse Growth Factor Receptor-Bound Protein 2, GRB2 ELISA Kit

Mouse Growth Factor Receptor-Bound Protein 2, GRB2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 11.66ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 11.66ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Growth Factor Receptor-Bound Protein 2

Code

E2656Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

11.66ng/L

Detection range

20-3800ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway. [Isoform 2]: Does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.

UniProt accession

MASS(Da)

25,238

GeneID

14784

Synonyms

Adapter protein GRB2;GRB 2;GRB2;Growth factor receptor-bound protein 2;SH2/SH3 adapter GRB2

Gene names

GRB2

Research area

Signal Transduction

Target protein

GRB2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2656Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 2.028 2.018 1.973
2.008
1000ng/L 1.485 1.464 1.419
1.443
500ng/L 0.667 0.627 0.582
0.587
250ng/L 0.445 0.44 0.395
0.436
125ng/L 0.176 0.187 0.142
0.199
0ng/L 0.053 0.045 0
0.037

Precision

We measured random samples of E2656Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1470.8 66.93 4.6
Intra-Assay 2 18 527.5 19.58 3.7
Intra-Assay 3 18 366.3 17.7 4.8

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2656Mo

    $320.00

  • 96T

    E2656Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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