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Home > Product > ELISA Kit > Porcine Endothelial Nitric Oxide Synthase, ENOS ELISA Kit

Porcine Endothelial Nitric Oxide Synthase, ENOS ELISA Kit

Porcine Endothelial Nitric Oxide Synthase, ENOS ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.55IU/mL

Detection range: 1-400IU/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product Summary

Size: 48T,96T

Sensitivity: 0.55IU/mL

Detection range: 1-400IU/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product overview

Full product name

Endothelial Nitric Oxide Synthase

Code

E0231Po

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.55IU/mL

Detection range

1-400IU/mL

Sample type

Serum, plasma, cell culture supernates

Species

Porcine

Storage

2-8ºC

Assay time

1h 30m

Background

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets (By similarity).

UniProt accession

MASS(Da)

133,406

GeneID

397557

Synonyms

cNOS;Constitutive NOS;EC-NOS;Endothelial NOS;eNOS;Nitric oxide Synthase, endothelial;NOS 3;NOS type III;NOS3;NOSIII

Gene names

NOS3

Research area

Neuroscience

Target protein

NOS3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0231Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
240IU/mL 2.289 2.177 2.13
2.064
120IU/mL 1.183 1.221 1.175
1.26
60IU/mL 0.856 0.77 0.723
0.683
30IU/mL 0.483 0.472 0.426
0.462
15IU/mL 0.241 0.247 0.201
0.253
0IU/mL 0.04 0.046 0
0.052

Precision

We measured random samples of E0231Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 35.2 2.52 7.1
Intra-Assay 2 18 88.9 5.34 6
Intra-Assay 3 18 161.4 5.6 3.5

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0231Po

    $320.00

  • 96T

    E0231Po

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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