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Home > Product > ELISA Kit > Human Pleiotrophin, PTN ELISA Kit

Human Pleiotrophin, PTN ELISA Kit

Human Pleiotrophin, PTN ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.026ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.026ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Pleiotrophin

Code

E0162Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.026ng/ml

Detection range

0.05-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Secreted growth factor that mediates its signal through cell-surface proteoglycan and non-proteoglycan receptors (PubMed: 16814777, PubMed: 11278720, PubMed: 19141530). Binds cell-surface proteoglycan receptor via their chondroitin sulfate (CS) groups (PubMed: 26896299, PubMed: 27445335). Thereby regulates many processes like cell proliferation, cell survival, cell growth, cell differentiation and cell migration in several tissues namely neuron and bone (PubMed: 1733956, PubMed: 1768439, PubMed: 11278720, PubMed: 19141530, PubMed: 27445335, PubMed: 30667096, PubMed: 19442624). Also plays a role in synaptic plasticity and learning-related behavior by inhibiting long-term synaptic potentiation (By similarity). Binds PTPRZ1, leading to neutralization of the negative charges of the CS chains of PTPRZ1, inducing PTPRZ1 clustering, thereby causing the dimerization and inactivation of its phosphatase activity leading to increased tyrosine phosphorylation of each of the PTPRZ1 substrates like ALK, CTNNB1 or AFAP1L2 in order to activate the PI3K-AKT pathway (PubMed: 17681947, PubMed: 27445335, PubMed: 30667096, PubMed: 16814777, PubMed: 10706604). Through PTPRZ1 binding controls oligodendrocyte precursor cell differentiation by enhancing the phosphorylation of AFAP1L2 in order to activate the PI3K-AKT pathway (PubMed: 27445335, PubMed: 30667096). Forms a complex with PTPRZ1 and integrin alpha-V/beta-3 (ITGAV:ITGB3) that stimulates endothelial cell migration through SRC dephosphorylation and activation that consequently leads to ITGB3 'Tyr-773' phosphorylation (PubMed: 19141530). In adult hippocampus promotes dendritic arborization, spine development, and functional integration and connectivity of newborn granule neurons through ALK by activating AKT signaling pathway (By similarity). Binds GPC2 and chondroitin sulfate proteoglycans (CSPGs) at the neuron surface, leading to abrogation of binding between PTPRS and CSPGs and neurite outgrowth promotion (By similarity). Binds SDC3 and mediates bone formation by recruiting and attaching osteoblasts/osteoblast precursors to the sites for new bone deposition (By similarity). Binds ALK and promotes cell survival and cell proliferation through MAPK pathway activation (PubMed: 11278720). Inhibits proliferation and enhances differentiation of neural stem cells by inhibiting FGF2-induced fibroblast growth factor receptor signaling pathway (By similarity). Mediates regulatory mechanisms in normal hemostasis and in hematopoietic regeneration and in maintaining the balance of myeloid and lymphoid regeneration (By similarity). In addition may play a role in the female reproductive system, auditory response and the progesterone-induced decidualization pathway (By similarity).

UniProt accession

MASS(Da)

18,942

GeneID

5764

Synonyms

HBBM;HB-GAM;HBGF-8;HBNF;HBNF-1;Heparin-binding brain mitogen;Heparin-binding growth factor 8;Heparin-binding growth-associated molecule;Heparin-binding neurite outgrowth-promoting factor;Heparin-binding neurite outgrowth-promoting factor 1;OSF-1;Osteoblast-specific factor 1;Pleiotrophin;PTN

Gene names

PTN

Research area

Cancer

Target protein

PTN

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0162Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/ml 2.106 2.116 2.066
2.126
8ng/ml 1.274 1.354 1.304
1.433
4ng/ml 0.701 0.764 0.714
0.828
2ng/ml 0.547 0.478 0.428
0.41
1ng/ml 0.279 0.281 0.231
0.283
0ng/ml 0.058 0.05 0
0.042

Precision

We measured random samples of E0162Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.2 0.38 6.2
Intra-Assay 2 18 12.3 0.46 3.7
Intra-Assay 3 18 2 0.14 7

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 96T

    E0162Hu

    $458.00

  • 48T

    E0162Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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