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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0665Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2.108	2.161	2.082
	2.213
400ng/L	1.15	1.168	1.089
	1.185
200ng/L	0.557	0.571	0.492
	0.584
100ng/L	0.299	0.284	0.206
	0.27
50ng/L	0.179	0.169	0.091
	0.159
0ng/L	0.074	0.078	0
	0.082

We measured random samples of E0665Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	215	9.3	4.3
Intra-assay	2	18	520.3	16.9	3.2
Intra-assay	3	18	360.1	18.9	5.3

> Protocol Booklet

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