Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 wells * 8 detachable strips |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0665Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 800ng/L | 2.108 | 2.161 | 2.082 |
| 2.213 | |||
| 400ng/L | 1.15 | 1.168 | 1.089 |
| 1.185 | |||
| 200ng/L | 0.557 | 0.571 | 0.492 |
| 0.584 | |||
| 100ng/L | 0.299 | 0.284 | 0.206 |
| 0.27 | |||
| 50ng/L | 0.179 | 0.169 | 0.091 |
| 0.159 | |||
| 0ng/L | 0.074 | 0.078 | 0 |
| 0.082 |
Precision
We measured random samples of E0665Po within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 215 | 9.3 | 4.3 |
| Intra-assay | 2 | 18 | 520.3 | 16.9 | 3.2 |
| Intra-assay | 3 | 18 | 360.1 | 18.9 | 5.3 |
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