Porcine Scavenger Receptor Cysteine-rich Type 1 Protein M130, CD163 ELISA Kit

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Home > Product > ELISA Kit > Porcine Scavenger Receptor Cysteine-rich Type 1 Protein M130, CD163 ELISA Kit

Porcine Scavenger Receptor Cysteine-rich Type 1 Protein M130, CD163 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 24.97ng/mL

Detection range: 50-10000ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product Summary

Size: 48T,96T

Sensitivity: 24.97ng/mL

Detection range: 50-10000ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product overview

Full product name

Scavenger Receptor Cysteine-Rich Type 1 Protein M130

Code

E0602Po

Assay type

Sandwich

Size

48T,96T

Sensitivity

24.97ng/mL

Detection range

50-10000ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Porcine

Storage

2-8ºC

Assay time

1h 30m

Background

Involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1 (By similarity). May play a role in the process of infection of porcine monocytes/macrophages by African swine fever virus (ASFV). In case of porcine reproductive and respiratory syndrome virus (PRRSV), serves mediates virion attachment and plays a role in viral entry. After shedding, the soluble form (sCD163) may play an anti-inflammatory role.

UniProt accession

Q2VL90

MASS（Da）

120,456

GeneID

397031

Synonyms

CD 163;CD antigen CD163;CD163;Scavenger receptor cysteine-rich type 1 protein M130;sCD163;Soluble CD163

Gene names

CD163

Research area

Immunology

Target protein

CD163

Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E0602Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
6400ng/mL	2.358	2.329	2.27
	2.299
3200ng/mL	1.387	1.446	1.388
	1.505
1600ng/mL	0.785	0.872	0.813
	0.958
800ng/mL	0.496	0.506	0.447
	0.515
400ng/mL	0.299	0.289	0.23
	0.278
0ng/mL	0.053	0.059	0
	0.064

Precision

We measured random samples of E0602Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	862.6	55.01	6.4
Intra-Assay	2	18	4363.5	171.64	3.9
Intra-Assay	3	18	2354.6	125.52	5.3

Protocols

> Protocol Booklet

References

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Associated products

Datasheet

Size

Code

Price

48T

E0602Po

$320.00
96T

E0602Po

$458.00

Add to Cart

Lead time: Within two weeks

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