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Home > Product > ELISA Kit > Rabbit Caspase 3, CASP3 ELISA Kit

Rabbit Caspase 3, CASP3 ELISA Kit

Rabbit Caspase 3, CASP3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.518ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rabbit

Product Summary

Size: 48T,96T

Sensitivity: 0.518ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rabbit

Product overview

Full product name

Caspase 3

Code

E0324Rb

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.518ng/mL

Detection range

0.1-40ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rabbit

Storage

2-8ºC

Assay time

1h 30m

Background

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress.

UniProt accession

MASS(Da)

31,653

GeneID

100008840

Synonyms

Apopain;CASP 3;CASP3;CASP-3;Caspase-3;Caspase-3 subunit p12;Caspase-3 subunit p17;Cysteine protease CPP32

Gene names

CASP3

Research area

Cell Biology; Cancer; Metabolism

Target protein

CASP3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0324Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/mL 1.905 1.906 1.867
1.907
12ng/mL 0.902 0.959 0.92
1.017
6ng/mL 0.557 0.488 0.449
0.419
3ng/mL 0.161 0.192 0.153
0.224
1.5ng/mL 0.099 0.098 0.059
0.097
0ng/mL 0.033 0.039 0
0.045

Precision

We measured random samples of E0324Rb within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 13.5 0.64 4.8
Intra-Assay 2 18 19.4 0.54 2.8
Intra-Assay 3 18 8.4 0.36 4.3

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0324Rb

    $320.00

  • 96T

    E0324Rb

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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