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Home > Product > ELISA Kit > Rabbit Cyclooxygenase-1, COX-1 ELISA Kit

Rabbit Cyclooxygenase-1, COX-1 ELISA Kit

Rabbit Cyclooxygenase-1, COX-1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.13ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rabbit

Product Summary

Size: 48T,96T

Sensitivity: 0.13ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rabbit

Product overview

Full product name

Cyclooxygenase-1

Code

E0369Rb

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.13ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rabbit

Storage

2-8ºC

Assay time

1h 30m

Background

Dual cyclooxygenase and peroxidase in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate, with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates arachidonate (AA, C20:4(n-6)) to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide PGH2, the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons. Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells.

UniProt accession

MASS(Da)

69,076

GeneID

100009407

Synonyms

COX-1;Cyclooxygenase-1;PGH Synthase 1;PGHS-1;PHS 1;Prostaglandin G/H Synthase 1;Prostaglandin H2 Synthase 1;Prostaglandin-endoperoxide Synthase 1;PTGS 1;PTGS1

Gene names

PTGS1

Research area

Others

Target protein

PTGS1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0369Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/ml 2.136 2.195 2.133
2.254
16ng/ml 1.418 1.428 1.366
1.438
8ng/ml 0.855 0.836 0.773
0.816
4ng/ml 0.409 0.468 0.406
0.528
2ng/ml 0.225 0.226 0.164
0.227
0ng/ml 0.072 0.062 0
0.052

Precision

We measured random samples of E0369Rb within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 7.8 0.36 4.6
Intra-Assay 2 18 19.9 0.62 3.1
Intra-Assay 3 18 3.9 0.22 5.6

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0369Rb

    $320.00

  • 96T

    E0369Rb

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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