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Home > Product > ELISA Kit > Rat Mothers Against Decapentaplegic Homolog 7, SMAD7 ELISA Kit

Rat Mothers Against Decapentaplegic Homolog 7, SMAD7 ELISA Kit

Rat Mothers Against Decapentaplegic Homolog 7, SMAD7 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.025ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.025ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Mothers Against Decapentaplegic Homolog 7

Code

E0078Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.025ng/ml

Detection range

0.05-15ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

UniProt accession

MASS(Da)

46458

GeneID

81516

Synonyms

MAD homolog 7;Mothers against decapentaplegic homolog 7;Mothers against DPP homolog 7;SMAD 7;SMAD family member 7;Smad7

Gene names

SMAD7

Research area

Signal Transduction; Epigenetics and Nuclear Signaling

Target protein

SMAD7

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0078Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8ng/ml 2.105 2.054 2.015
2.003
4ng/ml 1.359 1.439 1.4
1.519
2ng/ml 0.663 0.666 0.627
0.669
1ng/ml 0.348 0.386 0.347
0.423
0.5ng/ml 0.162 0.151 0.112
0.141
0ng/ml 0.021 0.039 0
0.057

Precision

We measured random samples of E0078Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2.1 0.1 4.6
Intra-Assay 2 18 4.2 0.17 4.1
Intra-Assay 3 18 1.4 0.06 4

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0078Ra

    $320.00

  • 96T

    E0078Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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