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Home > Product > ELISA Kit > Rat Cytokeratin 17, CK-17 ELISA Kit

Rat Cytokeratin 17, CK-17 ELISA Kit

Rat Cytokeratin 17, CK-17 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.02ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.02ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Cytokeratin 17

Code

E0091Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.02ng/ml

Detection range

0.05-10ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Type I keratin involved in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial 'stem cells'. Acts as a promoter of epithelial proliferation by acting a regulator of immune response in skin: promotes Th1/Th17-dominated immune environment contributing to the development of basaloid skin tumors. May act as an autoantigen in the immunopathogenesis of psoriasis, with certain Peptide regions being a major target for autoreactive T-cells and hence causing their proliferation.

UniProt accession

MASS(Da)

48,123

GeneID

287702

Synonyms

CK-17;Cytokeratin-17;K17;Keratin, type I cytoskeletal 17;Keratin-17;KRT 17;KRT17;Type I keratin Ka17

Gene names

KRT17

Research area

Others

Target protein

KRT17

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0091Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
6.4ng/ml 2.458 2.444 2.401
2.429
3.2ng/ml 1.43 1.427 1.384
1.423
1.6ng/ml 0.874 0.877 0.834
0.88
0.8ng/ml 0.402 0.414 0.372
0.426
0.4ng/ml 0.266 0.239 0.196
0.211
0ng/ml 0.043 0.042 0
0.041

Precision

We measured random samples of E0091Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1.6 0.08 4.7
Intra-Assay 2 18 3.8 0.17 4.5
Intra-Assay 3 18 1.2 0.06 4.9

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0091Ra

    $320.00

  • 96T

    E0091Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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